methods

from the cloning manuel by Sambrook

stock solution of 50x TAE buffer: electrophoresis buffer 242g of tris base 57.1 ml of glacial acetic acid 100 ml of 0.5M EDTA (pH 8.0) TAE yields a better resolution of DNA fragments.

working solution: 1x TAE 40mM Tris acetate 1mM EDTA (if making from scratch i believe)